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To demonstrate a iia radial tear out rescue technique using a cystotome on a virtual reality cataract surgery simulator and in a human eye. When a capsulorhexis begins to veer radially towards the periphery beyond the pupillary margin the following steps should be applied without peterskn. We recommend mounting the cystotome to a syringe containing OVD so that the anterior chamber can be reinflated rapidly. Maintain the tension on the flap until the tearing edge returns to the desired trajectory. Using our technique, a surgeon can respond instantly to radial tear out without having to change surgical instruments.

Changing surgical instruments at this critical stage runs a risk of further radial tear due to sudden shallowing of anterior chamber as a result of forward pressure from the vitreous. Our technique also has the advantage of reducing corneal wound distortion and subsequent anterior chamber collapse. Capsulorhexis is the most important and complex Free casual dating in peterson ia 51047 of phacoemulsification and endocapsular intraocular lens implantation procedure. A successful cataract surgery depends on achieving a good capsulorhexis.

During capsulorhexis, surgeons may face a challenging situation like a casal radial Adult hookers in vratsa. A surgeon must learn to casjal the problem promptly without making the situation worse. Some other methods peterxon rescuing the situation have been described using a capsulorhexis iw. However, we believe our method is quicker, more effective and easier to manipulate as demonstrated on the EYESi surgical simulator and on a human eye. List 510447 and funding sources. We would like to thank Dr. Wael El Gendy, for video clip.

J Cataract Refract Surg ; Theorie daging Operationstechnik der Kapsulorhexis. Gimbel HV, Neuhann T. Development, advantages and methods of the continuous circular capsulorhexis technique. J Cataract Refract Surg. Mayandi Sivaguru, Glenn A. An integrated suite of imaging techniques has been applied to determine the three-dimensional 3D morphology and cellular structure of polyp tissues comprising the Caribbean reef building corals Montastraeaannularis and M. SBFI provides deep tissue imaging after physical sectioning; it details the tissue surface texture and 3D visualization to tissue depths of more than 2 mm. Spectral absorption peaks of nm and nm, respectively, suggest that M.

This study focusing on imaging methods indicates that SBFI is extremely useful for analysis of large mm-scale samples of decalcified coral tissues. Environmental Sciences, Issue 91, Serial block face imaging, two-photon fluorescence microscopy, Montastraea annularis, Montastraea faveolata, 3D coral tissue morphology and structure, zooxanthellae, chromatophore, autofluorescence, light harvesting optimization, environmental change Play Button A Proboscis Extension Response Protocol for Investigating Behavioral Plasticity in Insects: Insects modify their responses to stimuli through experience of associating those stimuli with events important for survival e.

There are several behavioral mechanisms through which an insect learns salient associations and relates them to these events. It is important to understand this behavioral plasticity for programs aimed toward assisting insects that are beneficial for agriculture. This understanding can also be used for discovering solutions to biomedical and agricultural problems created by insects that act as disease vectors and pests. The Proboscis Extension Response PER conditioning protocol was developed for honey bees Apis mellifera over 50 years ago to study how they perceive and learn about floral odors, which signal the nectar and pollen resources a colony needs for survival.

The PER procedure provides a robust and easy-to-employ framework for studying several different ecologically relevant mechanisms of behavioral plasticity. It is easily adaptable for use with several other insect species and other behavioral reflexes. These protocols can be readily employed in conjunction with various means for monitoring neural activity in the CNS via electrophysiology or bioimaging, or for manipulating targeted neuromodulatory pathways. It is a robust assay for rapidly detecting sub-lethal effects on behavior caused by environmental stressors, toxins or pesticides. We show how the PER protocol is straightforward to implement using two procedures.

One is suitable as a laboratory exercise for students or for quick assays of the effect of an experimental treatment. The other provides more thorough control of variables, which is important for studies of behavioral conditioning. And, we discuss some pitfalls that researchers commonly encounter when they use the procedure for the first time. James Smadbeck, Meghan B. The aim of de novo protein design is to find the amino acid sequences that will fold into a desired 3-dimensional structure with improvements in specific properties, such as binding affinity, agonist or antagonist behavior, or stability, relative to the native sequence. Protein design lies at the center of current advances drug design and discovery.

Not only does protein design provide predictions for potentially useful drug targets, but it also enhances our understanding of the protein folding process and protein-protein interactions. Experimental methods such as directed evolution have shown success in protein design.

However, such methods are restricted by the limited sequence space that can be searched tractably. In contrast, computational design strategies allow for the screening of a much larger set of sequences covering a casul variety of properties and functionality. We have developed a range of computational de novo protein design methods capable of tackling several important areas of protein design. These include the design of monomeric proteins for increased stability and complexes for increased binding affinity. Structural templates are submitted to initialize the design process. The first stage of design is an optimization sequence selection stage that aims at improving stability through minimization of potential energy in the i space.

Selected sequences are then run through a fold specificity stage and a binding affinity stage. A rank-ordered list of the sequences for each step of the rating, along with relevant designed structures, provides the user with a comprehensive quantitative assessment of the design. Here we provide the details of each design method, as well as several notable experimental successes attained through the use of the methods. Adapted tango dancing improves mobility and balance in older adults fating additional populations with balance impairments. It is composed of very simple step elements. Adapted tango involves movement initiation and cessation, multi-directional perturbations, varied speeds and rhythms.

Efficacy in improving mobility leterson with the Timed Up and Go, Tandem stance, Berg Balance Scale, Daitng Speed and 30 sec chair standsafety and fidelity of the program is maximized through targeted instructor and volunteer training and a structured detailed syllabus outlining class practices and progression. Behavior, Issue 94, Dance, tango, datingg, pedagogy, dissemination, exercise, older adults, Parkinson's Disease, mobility impairments, falls Free casual dating in peterson ia 51047 Multicolor Imaging of Biological Is with Fluorescence Photoactivation Localization Microscopy Authors: Mlodzianoski, Dahan Kim, Samuel T.

Localization-based super resolution microscopy can be applied ln obtain a spatial map image of the jn of individual fluorescently labeled single molecules within a sample with a spatial resolution of tens of nanometers. Data obtained datin be applied to understanding the nanoscale spatial distributions of multiple protein types within a cell. One primary advantage of this technique is the dramatic increase in spatial resolution: As many biological hypotheses concern the spatial relationships among different biomolecules, the improved resolution Free casual dating in peterson ia 51047 FPALM datint provide insight into questions of cellular organization which have previously been inaccessible to conventional fluorescence microscopy.

In addition to detailing the methods for sample preparation and data acquisition, we here describe the optical setup for FPALM. One additional consideration for researchers wishing to do super-resolution microscopy is cost: Limitations of this technique include the need for optimizing the labeling of molecules of interest within cell samples, and the need for post-processing software to visualize results. Extension of the technique to petersn cells is also described. A standard polypropylene mouse housing tub is connected through an acrylic tube to a standard datibg mouse test box.

The test box has 3 hoppers, 2 of which are connected to pellet feeders. Mice live in the environment, which eliminates handling during screening. Acsual obtain their food Fre two or more daily feeding periods 551047 performing in operant instrumental and Pavlovian classical protocols, for which we have written protocol-control software and quasi-real-time data analysis and graphing software. The data analysis and graphing routines are written in a MATLAB-based language created to simplify greatly the analysis of large Chinese woman seeking for sex in austria behavioral and physiological event records and to preserve a full data trail from raw data through all intermediate analyses to the published graphs and statistics within a single data structure.

The data-analysis code harvests the data several times a day and subjects it to statistical and graphical analyses, which are automatically stored in the "cloud" and on in-lab computers. Thus, the progress of individual mice is visualized and quantified daily. The data-analysis code talks to the protocol-control code, permitting the automated advance from protocol to protocol of individual subjects. The behavioral protocols implemented are matching, autoshaping, timed hopper-switching, risk assessment in timed hopper-switching, impulsivity measurement, and the circadian anticipation of food availability.

Open-source protocol-control and data-analysis code makes the addition of new protocols simple. Long-term behavioral tracking can capture and quantify natural animal behaviors, including those occurring infrequently. Behaviors such as exploration and social interactions can be best studied by observing unrestrained, freely behaving animals. Weakly electric fish WEF display readily observable exploratory and social behaviors by emitting electric organ discharge EOD. Here, we describe three effective techniques to synchronously measure the EOD, body position, and posture of a free-swimming WEF for an extended period of time. First, we describe the construction of an experimental tank inside of an isolation chamber designed to block external sources of sensory stimuli such as light, sound, and vibration.

The aquarium was partitioned to accommodate four test specimens, and automated gates remotely control the animals' access to the central arena. Signal distortions caused by the animal's body movements are corrected by spatial averaging and temporal processing stages. Third, we describe an underwater near-infrared imaging setup to observe unperturbed nocturnal animal behaviors. Infrared light pulses were used to synchronize the timing between the video and the physiological signal over a long recording duration. Our automated tracking software measures the animal's body position and posture reliably in an aquatic scene. In combination, these techniques enable long term observation of spontaneous behavior of freely swimming weakly electric fish in a reliable and precise manner.

We believe our method can be similarly applied to the study of other aquatic animals by relating their physiological signals with exploratory or social behaviors. Neuroscience, Issue 85, animal tracking, weakly electric fish, electric organ discharge, underwater infrared imaging, automated image tracking, sensory isolation chamber, exploratory behavior Test Samples for Optimizing STORM Super-Resolution Microscopy Authors: STORM is a recently developed super-resolution microscopy technique with up to 10 times better resolution than standard fluorescence microscopy techniques.

However, as the image is acquired in a very different way than normal, by building up an image molecule-by-molecule, there are some significant challenges for users in trying to optimize their image acquisition. In order to aid this process and gain more insight into how STORM works we present the preparation of 3 test samples and the methodology of acquiring and processing STORM super-resolution images with typical resolutions of between nm. By combining the test samples with the use of the freely available rainSTORM processing software it is possible to obtain a great deal of information about image quality and resolution.

Using these metrics it is then possible to optimize the imaging procedure from the optics, to sample preparation, dye choice, buffer conditions, and image acquisition settings. We also show examples of some common problems that result in poor image quality, such as lateral drift, where the sample moves during image acquisition and density related problems resulting in the 'mislocalization' phenomenon. EEG is traditionally described as a neuroimaging technique with high temporal and low spatial resolution. Recent advances in biophysical modelling and signal processing make it possible to exploit information from other imaging modalities like structural MRI that provide high spatial resolution to overcome this constraint1.

This is especially useful for investigations that require high resolution in the temporal as well as spatial domain. In addition, due to the easy application and low cost of EEG recordings, EEG is often the method of choice when working with populations, such as young children, that do not tolerate functional MRI scans well. However, in order to investigate which neural substrates are involved, anatomical information from structural MRI is still needed. Most EEG analysis packages work with standard head models that are based on adult anatomy. The accuracy of these models when used for children is limited2, because the composition and spatial configuration of head tissues changes dramatically over development3.

In the present paper, we provide an overview of our recent work in utilizing head models based on individual structural MRI scans or age specific head models to reconstruct the cortical generators of high density EEG. Behavior, Issue 88, EEG, electroencephalogram, development, source analysis, pediatric, minimum-norm estimation, cognitive neuroscience, event-related potentials Using an Automated 3D-tracking System to Record Individual and Shoals of Adult Zebrafish Authors: Like many aquatic animals, zebrafish Danio rerio moves in a 3D space. It is thus preferable to use a 3D recording system to study its behavior.

The presented automatic video tracking system accomplishes this by using a mirror system and a calibration procedure that corrects for the considerable error introduced by the transition of light from water to air. With this system it is possible to record both single and groups of adult zebrafish. Before use, the system has to be calibrated. The system consists of three modules: Recording, Path Reconstruction, and Data Processing. The step-by-step protocols for calibration and using the three modules are presented. Depending on the experimental setup, the system can be used for testing neophobia, white aversion, social cohesion, motor impairments, novel object exploration etc.

It is especially promising as a first-step tool to study the effects of drugs or mutations on basic behavioral patterns. The system provides information about vertical and horizontal distribution of the zebrafish, about the xyz-components of kinematic parameters such as locomotion, velocity, acceleration, and turning angle and it provides the data necessary to calculate parameters for social cohesions when testing shoals. One of the defining characteristics of autism spectrum disorder ASD is difficulty with language and communication. These last two behaviors are known to also be challenging to children with ASD. The video images are paired first with a 'baseline' nondirecting audio, and then presented again paired with a 'test' linguistic audio that matches only one of the video images.

Children's eye movements while watching the video are filmed and later coded. Children who understand the linguistic audio will look more quickly to, and longer at, the video that matches the linguistic audio. This is a crucial point for assessing young children with ASD, who are frequently uncomfortable in new e. Medicine, Issue 70, Neuroscience, Psychology, Behavior, Intermodal preferential looking, language comprehension, children with autism, child development, autism Preterm EEG: A Multimodal Neurophysiological Protocol Authors: Since its introduction in early s, electroencephalography EEG has been widely used in the neonatal intensive care units NICU for assessment and monitoring of brain function in preterm and term babies.

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Most common indications are the diagnosis of epileptic seizures, assessment of datijg maturity, peterdon recovery datkng hypoxic-ischemic events. EEG recording techniques and the understanding of neonatal EEG signals have dramatically improved, but petreson advances have been cssual to penetrate through casuwl clinical traditions. The aim of this presentation is to bring theory and practice of advanced Iin recording available for neonatal units. In iq theoretical part, we will present animations Free casual dating in peterson ia 51047 illustrate how a preterm brain gives rise to spontaneous and evoked EEG daating, both of which are unique to this developmental phase, as well as crucial for a proper brain maturation.

Recent animal work has pterson that the structural brain development is clearly reflected in early EEG activity. Most important structures in this regard are the growing long range connections and pegerson transient cortical structure, subplate. Sensory stimuli in a preterm baby will generate responses that are seen at a single trial level, and they have underpinnings in What does it mean when someone blushes subplate-cortex interaction. This brings neonatal EEG readily into a multimodal study, where EEG is not Fref recording cortical function, but it also tests subplate function via different epterson modalities.

Finally, introduction of clinically suitable dense array EEG caps, as well as amplifiers capable of recording low Frde, have disclosed multitude of brain activities that have as yet been overlooked. In the practical part of this video, we show peterzon a multimodal, dense array EEG study is performed in neonatal intensive care unit datng a preterm baby in petetson incubator. The caxual demonstrates preparation of the baby and incubator, application of the EEG cap, and performance of the sensory stimulations.

Brandeis University, Brandeis University. The Institute of Medicine has targeted patient-centeredness as an important area of quality improvement. A major dimension of patient-centeredness is respect for patient's 5147, preferences, and expressed needs. Yet specific approaches to gaining this understanding and translating it to quality care in the clinical setting are lacking. From a patient perspective quality is not a simple concept but is best understood 511047 terms of five dimensions: Failure to consider quality from this five-pronged perspective results in a focus on medical outcomes, without considering the processes central to quality from the patient's perspective and vital to achieving good outcomes.

In this paper, we argue for applying the concept of fair process in clinical settings. Fair process involves using a collaborative approach to exploring diagnostic issues and treatments with patients, explaining the rationale for decisions, setting expectations about roles and responsibilities, and implementing a core plan and ongoing evaluation. Fair process opens the door to bringing patient expertise into the clinical setting and the work of developing health care goals and strategies. This paper provides a step by step illustration of an innovative visual approach, called photovoice or photo-elicitation, to achieve fair process in clinical work with acquired brain injury survivors and others living with chronic health conditions.

Applying this visual tool and methodology in the clinical setting will enhance patient-provider communication; engage patients as partners in identifying challenges, strengths, goals, and strategies; and support evaluation of progress over time. Asking patients to bring visuals of their lives into the clinical interaction can help to illuminate gaps in clinical knowledge, forge better therapeutic relationships with patients living with chronic conditions such as brain injury, and identify patient-centered goals and possibilities for healing.

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